Journal: Frontiers in Genetics

Article Title: Integrated Bioinformatics Analysis Identifies Heat Shock Factor 2 as a Prognostic Biomarker Associated With Immune Cell Infiltration in Hepatocellular Carcinoma

doi: 10.3389/fgene.2021.668516

Figure Lengend Snippet: HSF2 mediates the proliferation and malignancy of HCC cells. (A) The knockdown efficacy was confirmed by western blotting. (B) Cell viability was examined using a CCK-8 assay. (C) The migration of HepG2 and Hep3B cells was detected using a wound healing assay. Scale bar, 100 μm. (D) Representative images of colonies formed by HepG2 and Hep3B cells. (E) The invasive ability of HepG2 and Hep3B cells with HSF2 knockdown was detected by performing Matrigel-coated Transwell assay. Numbers of invaded cells were counted. Scale bar, 100 μm. (F) Scatterplots showing the correlations of HSF2 expression and CDH2, VIM, SNAI1, SNAI2, MMP2 and MMP9 in HCC using the GEPIA2 database. Data are presented as the means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.01.

Article Snippet: Briefly, the upper Transwell inserts were coated with 100 μl of Matrigel basement membrane (BD Biosciences, San Diego, CA, United States), and then 100 μl of serum-free DMEM medium was added.

Techniques: Western Blot, CCK-8 Assay, Migration, Wound Healing Assay, Transwell Assay, Expressing